Regulatory

Part:BBa_K3154003:Design

Designed by: iGEM19_SASTRA_Thanjavur   Group: iGEM19_SASTRA_Thanjavur   (2019-10-16)


Second Generation Toehold Switch for hsa-miR-20a-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design constraints for the toehold switch were optimized using machine learning techniques. The dynamic range of the toehold switch, or, the ratio of the largest signal value to the smallest signal value of GFP expression, is the best performance metric for the toehold switch. The first attempt at toehold switch design was validated by a multivariate regression model and the second was using a multilayer perceptron. The toehold switches are efficacious regulatory elements. The performance of the switches is best when the toehold domain is long, at about 11-12 nucleotides, the number of GC base pairs at zero or one in the top stem, two to four in the bottom stem, large primary loop around 12 nucleotides containing the RBS, longer bottom stems for increased stability and an array of free energy features that determine that dynamic range of the switch. Our part consists of an Anderson RBS sequence (strong RBS sequence)



Source

This part does not have any genomic origin.

References